In case of non-compliance, follow the Maintenance Program.ĬHECKPOINT: D2 LAMP ENERGY CHECK (DETECTOR)Ģ. Prepare Calibration Status Label and display on the instrument at the designated place.ġ2. Compare the result for its compliance with the limit given in the Calibration Log and put the remark regarding the Calibration Status.ġ1. Find out the Correlation coefficient “r2” for each peak at five levels and record in the Calibration Log.ġ0. Plot the curve for the area corresponding to Benzene to Toluene peaks v/s injection volume, Find out the RSD (reproducibility) and record in the Calibration Log.ĩ. Make entry of the usage of the instrument and HPLC column in the Instrument Usage Log Register and Column Usage Log Register respectively.Ĩ. Record the Area and Retention times of the of the Benzene and Toluene peaks in the Calibration Log.ħ. Take the print out of the chromatograms and attach to the Calibration Log.Ħ. Repeat the injection of the above solution by injecting 15, 20, 25, 30 µl in triplicate.ĥ. Follow the Instrument Operating procedure, Inject 10 µl in triplicate and record the chromatograms.Ĥ. Ensure that, the instrument is set according to the Chromatographic conditions.ģ. Make up the volume to 50 ml with Methanol and mix well. Pipette out 1.0 ml of Benzene and Toluene into the clean and dry 50 ml volumetric flask.ģ. Take clean and dry 50 ml volumetric flask.Ģ. REPRODUCIBILITY AND LINEARITY OF INJECTION VOLUMEġ. In case of non-compliance, follow the Maintenance Program. Prepare Calibration Status Label and display on the instrument at the designated place.ġ1. Make entry of the usage of the instrument and HPLC column in the Instrument Usage Log Register and Column Usage Log Register respectively.ġ0. Compare the results for its compliance with limits given in the Calibration Log and put the remark regarding the Calibration Status.ĩ. Repeat step 3 to 6 but using methanol HPLC grade as mobile phase instead of water.Ĩ. Repeat the procedure for 1.0 ml, 1.5 ml and 2.0 ml/ min. Record the time required to collect the 10 ml mobile phase in the calibration log.Ħ. Wait till the collected mobile phase reaches 10 ml mark of the volumetric flask and Stop the stopwatch.ĥ. Keep the Drain tube in such a way that the mobile phase (Water) drops falls into 10 ml clean, dry volumetric flask without touching the walls of the flask and start immediately the stopwatch when the first drop falls into the flask.Ĥ. Ensure that, the Pump is passing the “Leakage Test (By Pressure Drop)”.ģ. Ensure that, the instrument is ready for calibration and Start-up procedure is followed.Ģ. Related: Principle and Working of HPLC Chromatographyġ.
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